Understanding the Heat Shock Response in the Sea Cucumber Apostichopus japonicus, Using iTRAQ-Based Proteomics.

Mortality of the sea cucumber, Apostichopus japonicus Selenka, exposed to acute salinity decrease and related physiological responses: Osmoregulation and heat shock protein expression

Heat stress-mediated gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus

Suppression subtractive hybridization was used to analyze the differences in gene expression in the body wall of the Japanese sea cucumber Apostichopus japonicus, induced by heat stress (28°C), in order to understand the response at the molecular level. A total of 737 clones was selected and analyzed, and 165 distinct sequences were obtained, with 65 sharing similarity to sequences in public da...

iTRAQ-Based Proteomics Reveals Novel Members Involved in Pathogen Challenge in Sea Cucumber Apostichopus japonicus

Skin ulceration syndrome (SUS) is considered to be a major constraint for the stable development of Apostichopus japonicus culture industries. In this study, we investigated protein changes in the coelomocytes of A. japonicus challenged by Vibrio splendidus using isobaric tags for relative and absolute quantification (iTRAQ) over a 96 h time course. Consequently, 228 differentially expressed pr...

Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus

To evaluate the thermal resistance of marine invertebrates to elevated temperatures under scenarios of future climate change, it is crucial to understand parental effect of long acclimatization on thermal tolerance of offspring. To test whether there is parental effect of long acclimatization, adult sea cucumbers (Apostichopus japonicus) from the same broodstock were transplanted southward and ...

Difference in thermotolerance between green and red color variants of the Japanese sea cucumber, Apostichopus japonicus Selenka: Hsp70 and heat-hardening effect.

We studied thermal tolerance limits, heat-hardening, and Hsp70 to elucidate the difference in thermotolerance between two color variants of the sea cucumber Apostichopus japonicus. Green and Red variants occupy different habitats and have different aestivation responses to high temperature in summer. In the absence of heat-hardening, the variants showed no difference in the temperature at which...